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KMID : 0387720100210010074
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Korean Journal of Blood Transfusion
2010 Volume.21 No. 1 p.74 ~ p.79
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Auto-antibody Showing Anti-Fyb Specificity as Proven by the Dilution Method in the Presence of Warm Autoantibodies: A Case Report
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Jeong Eun-Sun
Cho Duck
Ryang Dong-Wook
Lim Chae-Seung
Jang Min-Joong
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Abstract
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Several approaches have been introduced to detect allo-antibodies in the presence of warm auto-antibodies, and these methods include warm autoadsorption, cysteine-activated papain and dithiothreitol (ZZAP), and polyethylene glycol (PEG) and dilution of the patient’s serum. Among them, the dilution technique is a simple and rapid method. During pretransfusion testing of a 33 year-old systemic lupus erythematosus (SLE) patient with warm auto-antibodies, antibody identification was done by the dilution technique with using serum diluted 1-in-8. The patient demonstrated an anti-Fyb pattern of reactivity in his sera. Contrary to our expectations, the phenotype of the erythrocytes was Fy(a+/b+) and the genotype, as assessed by performing PCR-restriction fragment length polymorphism (RFLP), was FY*A/FY*B. These results suggest that the antibody is an autoantibody showing anti-Fyb specificities. An antibody identification test using undiluted serum showed the same result when 40 days had passed. We report here on a case with auto-anti-Fyb proven by the dilution method in the presence of warm autoantibodies.
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KEYWORD
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Anti-Fyb, Dilution method, Warm autoantibody
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